Joint effect of traveling wave dielectrophoresis and AC electroosmotic fluid flow is used to sort bacteria from other particles and increase the bacteria output concentration in a microfluidic device. The device consists of a thin and long rectangular channel with two interdigitated electrode arrays, one at the bottom and one at the top of the channel, that are used to generate a nonuniform electric field. A four-phase signal at high frequency superposed on a low frequency signal is applied. At the end of the channel, the fluid is collected in two outputs: the bacteria are collected on one side and fluid without bacteria is collected on the other side. We have previously demonstrated a method to optimize cell separation using multiple frequency dielectrophoresis. The device presented here illustrates a novel use of multiple frequencies that permits the combined use of traveling wave dielectrophoresis and AC electroosmotic fluid flow.

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