In this paper, we are presenting the numerical simulation of a novel, simple microfluidic device for continuous separation of the particles according to their size. The device is composed of a straight rectangular channel connecting two inlet reservoirs to two exit reservoirs. Two asymmetric, straight, 3D electrodes are embedded inside the channel along the wall to create a non-uniform electric field for the DEP separation. The separated particles are collected at the different exit reservoirs. Main flow is induced by the pressure difference between the inlet and the exit reservoirs. The region affected by the electric field gradient is confined within the vicinity of the electrodes. Therefore, the undesired effects of the electric field on the system are minimized.

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