A microfluidic device with an embedded surface plasmon resonance (SPR) sensor array was developed. The sensing elements were nanohole arrays, differentiated by periodicity. The device was applied to detection of concentration across a gradient, and the detection of surface binding events. To determine sensitivity and for the gradient-based detection experiments, glucose solutions of known refractive index were employed. Consecutive surface binding events were monitored in the flow-through assembly of a monolayer (cysteamine) with a linker (biotin) and a protein (streptavidin).

Volume Subject Area:
Fluids Engineering
Topics:
Plasmons (Physics), Sensors, Flow (Dynamics), Glucose, Manufacturing, Microfluidics, Proteins, Refractive index, Surface plasmon resonance
1.
Barnes
W. L.
,
Dereux
A.
, and
Ebbesen
T. W.
, “
Surface plasmon subwavelength optics
,”
Nature
,
424
, pp
824
–
830
,
2003
.
2.
A. De Leebeeck, D. Sinton, A.G. Brolo, E. Arctander, R. Gordon, and K.L. Kavanagh, Electrokinetically-induced flow over a nano-hole array sensor,” ASME-IMECE2004-61606, Anaheim, CA, Nov. 2004.
3.
Brolo
A. G.
,
Gordon
R.
,
Leathem
B.
, and
Kavanagh
K. L.
, “
Surface plasmon sensor based on the enhanced light transmission through arrays of nanoholes in gold films
,”
Langmuir
,
20
, pp.
4813
–
4815
,
2004
.
4.
Brolo
A. G.
,
Kwok
S. C.
,
Moffitt
M. G.
,
Gordon
R.
,
Riordon
J.
, and
Kavanagh
K. L.
, “
Enhanced Fluorescence from Arrays of Nanoholes in a Gold Film
,”
Journal of the American Chemical Society
,
127
, pp
14936
–
14941
,
2005
.
5.
McDonald
 et al. “
Fabrication of microfluidic systems in poly(dimethylsiloxane)
,”
Electrophoresis
,
21
, pp
27
–
40
,
2000
.
This content is only available via PDF.
Copyright © 2006
 by ASME
You do not currently have access to this content.