This paper describes the results of fabricating plastic microfluidic devices and creating a microvalve array for protein separation. Plastic devices are selected due to low cost of raw materials, bio-compatibility, and disposability. Although the methods for fabricating plastic devices have appeared in literature, reports typically indicate one set of conditions that yield functional devices. We report a systematic study of fabrication process parameters including compression rate, molding temperature, and the compression force used by a hydraulic press. Their effects on the device thickness, channel dimension, and pattern transfer fidelity will be discussed. In addition, we investigated creating an array of pseudo-microvalves using photodefinable, in situ gel polymerization. The valves were developed for introducing two types of separation media for performing two-dimensional protein separation in a microfluidic device. We also demonstrated rapid protein separation using the mechanism for the first dimension, isoelectric focusing.
Skip Nav Destination
ASME 2005 International Mechanical Engineering Congress and Exposition
November 5–11, 2005
Orlando, Florida, USA
Conference Sponsors:
- Microelectromechanical Systems Division
ISBN:
0-7918-4224-X
PROCEEDINGS PAPER
Fabricating Plastic Microfluidic Devices With Photodefinable Microvalves for Protein Separations
Carl K. Fredrickson,
Carl K. Fredrickson
University of Florida
Search for other works by this author on:
Fernando T. Tavares,
Fernando T. Tavares
University of Florida
Search for other works by this author on:
Z. Hugh Fan
Z. Hugh Fan
University of Florida
Search for other works by this author on:
Carl K. Fredrickson
University of Florida
Champak Das
University of Florida
Fernando T. Tavares
University of Florida
Ryan Ferguson
University of Florida
Zheng Xia
University of Florida
Z. Hugh Fan
University of Florida
Paper No:
IMECE2005-79229, pp. 3-7; 5 pages
Published Online:
February 5, 2008
Citation
Fredrickson, CK, Das, C, Tavares, FT, Ferguson, R, Xia, Z, & Fan, ZH. "Fabricating Plastic Microfluidic Devices With Photodefinable Microvalves for Protein Separations." Proceedings of the ASME 2005 International Mechanical Engineering Congress and Exposition. Microelectromechanical Systems. Orlando, Florida, USA. November 5–11, 2005. pp. 3-7. ASME. https://doi.org/10.1115/IMECE2005-79229
Download citation file:
7
Views
Related Proceedings Papers
Related Articles
A New Protein Sensor Platform Based on Competitive Protein Adsorption for Thyroglobulin Detection
J. Med. Devices (June,2009)
Molecular Imprinted Polymer for a Purfication Device
J. Med. Devices (June,2009)
Development of Fieldable Lab-on-a-Chip Systems for Detection of a Broad Array of Targets From Toxicants to Biowarfare Agents
J. Nanotechnol. Eng. Med (May,2013)
Related Chapters
Incremental Model Adjustment
Nonlinear Regression Modeling for Engineering Applications: Modeling, Model Validation, and Enabling Design of Experiments
Pressure Molding Factors of Mineral Licking Brick
International Conference on Information Technology and Management Engineering (ITME 2011)
Structure, Properties, and Applications of Plastics
Introduction to Plastics Engineering