This work investigated the electrokinetic focusing and dispensing of polystyrene particles and red blood cells on microfluidic chips. Particles or cells were first electrokinetically focused using the merging of focusing streams on the sample stream, and subsequently separated as a result of the focusing. These particles or cells were then selectively dispensed from the focused sample stream using precise application of electrical pulses. The whole process of focusing, separation and dispensing of particles was visualized by a custom-made microscopy system. In particular, the width of the focused fluorescein stream and the accelerated electrophoretic motion of particles and cells were measured in a cross-channel and compared with a proposed analytical model. The electrokinetic manipulation of particles and cells demonstrated in this work can be used for developing integrated lab-on-a-chip devices for studies of cells.
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ASME 2005 International Mechanical Engineering Congress and Exposition
November 5–11, 2005
Orlando, Florida, USA
Conference Sponsors:
- Microelectromechanical Systems Division
ISBN:
0-7918-4224-X
PROCEEDINGS PAPER
Electrokinetic Focusing and Dispensing of Particles and Cells on Microfluidic Chips Available to Purchase
Edmond W. K. Young,
Edmond W. K. Young
University of Toronto
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Dongqing Li
Dongqing Li
University of Toronto
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Xiangchun Xuan
University of Toronto
Edmond W. K. Young
University of Toronto
Dongqing Li
University of Toronto
Paper No:
IMECE2005-80223, pp. 213-219; 7 pages
Published Online:
February 5, 2008
Citation
Xuan, X, Young, EWK, & Li, D. "Electrokinetic Focusing and Dispensing of Particles and Cells on Microfluidic Chips." Proceedings of the ASME 2005 International Mechanical Engineering Congress and Exposition. Microelectromechanical Systems. Orlando, Florida, USA. November 5–11, 2005. pp. 213-219. ASME. https://doi.org/10.1115/IMECE2005-80223
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