This paper presents a novel microfluidic photomasking strategy. Two opaque, dyed streams hydrodynamically focus a transparent stream to define a line of light transmission. Aligning two separate chips orthogonal to each other defines a small area where the transparent streams overlap. A proof-of-concept chip was developed and tested using visible-spectrum light and a visible light absorbing dye. Probe spot sizes of ~80μm are attainable, with spacing between spots of ~150μm.

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