The poor intrinsic healing capacity of articular cartilage has led to a number of attempts to engineer a replacement tissue [1]. One of these approaches, termed functional tissue engineering, suggests that the application of mechanical preconditioning, mimicking the in vivo loading environment, may enhance the development of material properties in these constructs [2,3]. Using this approach, our previous studies have demonstrated that dynamic loading (DL) increases the mechanical properties of chondrocyte-seeded agarose hydrogels relative to free swelling (FS) controls [4–6]. One mechanism by which the increase in mechanical properties occurs is hypothesized to be due to enhanced transport of nutrients and/or growth factors under dynamic loading [7]. The goal of the current study is to determine the effect of dynamic loading on the transport of neutral dextran molecules into agarose gels. Dextran, a neutral and generally inert solute commonly used in diffusion and transport studies, is used in its fluorophore-conjugated form thus making it possible to track the solute and quantify its content inside a hydrogel. We hypothesize that the uptake of dextran molecules into the agarose gels will be significantly enhanced under the influence of physiological dynamic deformation loading. Two varying molecular weights of dextran, 3 kDa and 70 kDa, were chosen in this study to ascertain a wide range of transport behaviors, and to interpret the experimental results in the context of a recently developed mixture theory model for the transport of neutral solutes in a neutrally charged gel, such as agarose [8].
Skip Nav Destination
ASME 2004 International Mechanical Engineering Congress and Exposition
November 13–19, 2004
Anaheim, California, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-4703-9
PROCEEDINGS PAPER
Effect of Dynamic Deformational Loading on the Transport of Dextran Molecules Into Agarose Gels Available to Purchase
Gerard A. Ateshian
Gerard A. Ateshian
Columbia University
Search for other works by this author on:
Nadeen O. Chahine
Columbia University
Eric G. Lima
Columbia University
Clark T. Hung
Columbia University
Gerard A. Ateshian
Columbia University
Paper No:
IMECE2004-61368, pp. 401-402; 2 pages
Published Online:
March 24, 2008
Citation
Chahine, NO, Lima, EG, Hung, CT, & Ateshian, GA. "Effect of Dynamic Deformational Loading on the Transport of Dextran Molecules Into Agarose Gels." Proceedings of the ASME 2004 International Mechanical Engineering Congress and Exposition. Advances in Bioengineering. Anaheim, California, USA. November 13–19, 2004. pp. 401-402. ASME. https://doi.org/10.1115/IMECE2004-61368
Download citation file:
12
Views
Related Proceedings Papers
Related Articles
Parametric Finite Element Analysis of Physical Stimuli Resulting From Mechanical Stimulation of Tissue Engineered Cartilage
J Biomech Eng (June,2009)
Related Chapters
Effects of Ultrasound Stimulation on Chondrocytes in Three-Dimensional Culture in Relation to the Production of Regenerative Cartilage Tissue
Biomedical Applications of Vibration and Acoustics in Therapy, Bioeffect and Modeling
Synthesis and Characterization of Carboxymethyl Chitosan Based Hybrid Biopolymer Scaffold
International Conference on Mechanical and Electrical Technology, 3rd, (ICMET-China 2011), Volumes 1–3
On the Evaluation of Thermal and Mechanical Factors in Low-Speed Sliding
Tribology of Mechanical Systems: A Guide to Present and Future Technologies