A planar waveguide (PWG) device has been developed for the detection of fluorescently labeled nucleic acid sequences immobilized or hybridized to the surface of a planar waveguide. Unlike current technologies requiring image gathering and reading capabilities or specially textured waveguide surfaces, this instrument uses simple glass slide based arrays, providing a numerical output in proportion to the fluorescent intensity recorded. The system consists of an optical waveguide (a glass microscope slide), an excitation light source, a photo detector, filters for select fluorescent emissions and the positioning array cassette. A data analysis algorithm is presented for interpretation of two dimensionally organized arrays. Based on our experimental evaluations we conclude that this sensing system shows promise as a simple and effective means to read fluorescent microarrays.
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ASME 2003 International Mechanical Engineering Congress and Exposition
November 15–21, 2003
Washington, DC, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-3710-6
PROCEEDINGS PAPER
Planar Waveguide Genetic Assay Readout Device
David R. Wulfman,
David R. Wulfman
University of Minnesota, Minneapolis, MN
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Tracey L. Baas,
Tracey L. Baas
University of Minnesota, Minneapolis, MN
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Ronald C. McGlennen
Ronald C. McGlennen
University of Minnesota, Minneapolis, MN
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David R. Wulfman
University of Minnesota, Minneapolis, MN
Tracey L. Baas
University of Minnesota, Minneapolis, MN
Ronald C. McGlennen
University of Minnesota, Minneapolis, MN
Paper No:
IMECE2003-43442, pp. 165-166; 2 pages
Published Online:
May 12, 2008
Citation
Wulfman, DR, Baas, TL, & McGlennen, RC. "Planar Waveguide Genetic Assay Readout Device." Proceedings of the ASME 2003 International Mechanical Engineering Congress and Exposition. Advances in Bioengineering. Washington, DC, USA. November 15–21, 2003. pp. 165-166. ASME. https://doi.org/10.1115/IMECE2003-43442
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