The Ratcheting Electrophoresis Microchip (REM) is a microfluidic device for electrophoretic separation of biomolecules such as DNA and proteins. By using thousands of electrodes along the length of a microchannel, the REM separates molecules using low applied voltages (∼1 V) in short times (< 1 minute). This paper describes the microfabriation of the REM and initial testing results. Parallel arrays of platinum electrodes are fabricated on a silicon chip with a pitch of 10 μm. Two types of channels are fabricated: silicon nitride channels fabricated on the chip and poly(dimelthylsiloxane) (PDMS) channels fabricated separately and attached to the chip. Initial testing shows partial success with the PDMS channels and promis ing results for the silicon nitride channels.
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ASME 2002 International Mechanical Engineering Congress and Exposition
November 17–22, 2002
New Orleans, Louisiana, USA
Conference Sponsors:
- Fluids Engineering Division
ISBN:
0-7918-3657-6
PROCEEDINGS PAPER
Rapid Separation and Manipulation of DNA by a Ratcheting Electrophoresis Microchip (REM)
Katherine Dunphy,
Katherine Dunphy
University of California at Berkeley, Berkeley, CA
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Veljko Milanovic,
Veljko Milanovic
University of California at Berkeley, Berkeley, CA
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Samantha Andrews,
Samantha Andrews
University of Florida, Gainesville, FL
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Arun Majumdar
Arun Majumdar
University of California at Berkeley, Berkeley, CA
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Katherine Dunphy
University of California at Berkeley, Berkeley, CA
Veljko Milanovic
University of California at Berkeley, Berkeley, CA
Samantha Andrews
University of Florida, Gainesville, FL
Taku Ohara
Tohoku University, Sendai, Japan
Arun Majumdar
University of California at Berkeley, Berkeley, CA
Paper No:
IMECE2002-33564, pp. 419-423; 5 pages
Published Online:
June 3, 2008
Citation
Dunphy, K, Milanovic, V, Andrews, S, Ohara, T, & Majumdar, A. "Rapid Separation and Manipulation of DNA by a Ratcheting Electrophoresis Microchip (REM)." Proceedings of the ASME 2002 International Mechanical Engineering Congress and Exposition. Fluids Engineering. New Orleans, Louisiana, USA. November 17–22, 2002. pp. 419-423. ASME. https://doi.org/10.1115/IMECE2002-33564
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