This paper presents an experimental investigation of field amplified sample stacking (FASS) with micron resolution particle image velocimetry (μPIV). The preliminary experiments reported in this work show particle velocity fields in electrokinetic flow in a glass microchannel with a single buffer-buffer interface. The buffer-to-buffer conductivity ratio is 10. Stacking of latex microspheres (i.e., increases in their number density) in the presence of a background electroosmotic flow is demonstrated. The generation of an internal pressure gradient is quantified using μPIV. This work is part of an ongoing study of the spatial and temporal development of the velocity and concentration profiles of FASS systems.

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