Cell volume regulation has been observed in almost all cell types examined to date. When cells are exposed to hypotonic solutions a quick increase in volume is followed by a more gradual return, termed regulatory volume decrease (RVD). The mechanism associated with RVD depends upon cell type and species, but in bovine chondrocytes the non-selective osmolyte channels are mainly responsible [1]. In a chondrocyte, volume control is critical for the maintenance of metabolism, and biosynthesis. Volume fluctuations can be due to changes in hydrostatic pressure, fluid flows, deformation, and extracellular matrix (ECM) hydration. Alterations in hydration can occur during static loading of articular cartilage or during the early stages of osteoarthritis [1], which have been correlated with changes in cellular metabolism. The swelling behaviour of chondrocytes, and the mechanism by which they sense and respond to changes in their physico-chemical environment, are not well understood [1]. To investigate the effects of osmotic environment on chondrocyte behaviour it is often beneficial to isolate cells from the ECM, which can be achieved by a variety of techniques. To investigate the effect of isolation technique on the swelling behaviour of bovine chondrocytes, two enzymatic digestion techniques were chosen for this study.
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ASME 2002 International Mechanical Engineering Congress and Exposition
November 17–22, 2002
New Orleans, Louisiana, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-3650-9
PROCEEDINGS PAPER
Enzymatic Digestion Technique Influences Regulatory Volume Decrease of Isolated Bovine Chondrocytes
Paul Hulme,
Paul Hulme
University of Calgary, Calgary, Alberta, Canada
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Simon Chi,
Simon Chi
University of Calgary, Calgary, Alberta, Canada
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Dominic Young,
Dominic Young
University of Calgary, Calgary, Alberta, Canada
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John Matyas,
John Matyas
University of Calgary, Calgary, Alberta, Canada
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Neil A. Duncan
Neil A. Duncan
University of Calgary, Calgary, Alberta, Canada
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Paul Hulme
University of Calgary, Calgary, Alberta, Canada
Simon Chi
University of Calgary, Calgary, Alberta, Canada
Dominic Young
University of Calgary, Calgary, Alberta, Canada
John Matyas
University of Calgary, Calgary, Alberta, Canada
Neil A. Duncan
University of Calgary, Calgary, Alberta, Canada
Paper No:
IMECE2002-32671, pp. 53-54; 2 pages
Published Online:
June 3, 2008
Citation
Hulme, P, Chi, S, Young, D, Matyas, J, & Duncan, NA. "Enzymatic Digestion Technique Influences Regulatory Volume Decrease of Isolated Bovine Chondrocytes." Proceedings of the ASME 2002 International Mechanical Engineering Congress and Exposition. Advances in Bioengineering. New Orleans, Louisiana, USA. November 17–22, 2002. pp. 53-54. ASME. https://doi.org/10.1115/IMECE2002-32671
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