Hypothermic machine perfusion preservation (MPP) has proven to be a successful technique for hypothermic kidney storage, however this technology has not successfully been applied to the liver. Recent research has indicated that the endothelial cells lining the liver sinusoids display rounding phenomena during MPP that is not fully understood. In order to gain a better understanding of endothelial cell shear stress response and the factors that induce rounding, a temperature-controlled micro-shear chamber has been designed and fabricated. The micro-shear chamber has been used to apply shear stresses, corresponding to those imposed during MPP, to rat liver primary endothelial cell cultures in order to form an understanding of how these stresses affect endothelial cell morphology. The chamber allows for the application of shear stresses ranging from 0.2 ± .01 dynes/cm2 to 2.3 ± 0.3 dynes/cm2, corresponding to what occurs during MPP.] Twenty-four hour in vitro experiments with shear stresses ranging from 0 to 1.49 dynes/cm2 at 4 °C were conducted in order to replicate in vivo conditions of the liver during hypothermic MPP. It has been demonstrated that endothelial cell rounding increases with increasing shear and can be prevented by utilizing low flow rates.
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ASME 2002 International Mechanical Engineering Congress and Exposition
November 17–22, 2002
New Orleans, Louisiana, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
0-7918-3650-9
PROCEEDINGS PAPER
The Effects of Shear Stress on Endothelial Cells at Hypothermic Temperatures
John H. Slater,
John H. Slater
University of North Carolina at Charlotte, Charlotte, NC
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Shailendra Jain,
Shailendra Jain
University of North Carolina at Charlotte, Charlotte, NC
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Robin N. Coger,
Robin N. Coger
University of North Carolina at Charlotte, Charlotte, NC
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Charles Y. Lee
Charles Y. Lee
University of North Carolina at Charlotte, Charlotte, NC
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John H. Slater
University of North Carolina at Charlotte, Charlotte, NC
Shailendra Jain
University of North Carolina at Charlotte, Charlotte, NC
Robin N. Coger
University of North Carolina at Charlotte, Charlotte, NC
Charles Y. Lee
University of North Carolina at Charlotte, Charlotte, NC
Paper No:
IMECE2002-33705, pp. 219-225; 7 pages
Published Online:
June 3, 2008
Citation
Slater, JH, Jain, S, Coger, RN, & Lee, CY. "The Effects of Shear Stress on Endothelial Cells at Hypothermic Temperatures." Proceedings of the ASME 2002 International Mechanical Engineering Congress and Exposition. Advances in Bioengineering. New Orleans, Louisiana, USA. November 17–22, 2002. pp. 219-225. ASME. https://doi.org/10.1115/IMECE2002-33705
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