The wear particles generated from a tire model made of vulcanized natural rubber were analyzed using ELISA (Enzyme-Linked Immunosorbent Assay). Antibodies to 4 different allergens were used to analyze the extracted proteins from the wear particles. Latex allergens were detected when the slip ratio was large. However, no clear relationship between the latex allergen content extracted from the wear debris and either the average size or the number of wear particles was observed.

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