A method to actively controlling crystallization is one of promising technique for cryopreservation. The object of this paper is to study the effect of ultrasonic irradiation on ice formation during freezing of biological tissue with supercooling. In the experiments, agar gel was frozen under irradiation of ultrasound at frequency of 28kHz. The measurements of temperature and the microscopic observation of ice crystals using fluorescent indicator were carried out. Firstly, it was found that the supercooled state was released by applying ultrasound at high intensity level, since ultrasonic cavitation effects were augmented. On the other hand, a weakly delay in the nucleation temperature was induced by applying ultrasound at low intensity level. Secondly, ice structure size decreased with increasing ultrasonic power under given supercooling degree at nucleation stage. The effect of ultrasound was enhanced by using large supercooling condition. Thirdly, paying attention to the thermal effect of ultrasound as changing temperature profile, sound and heat conduction in agar gel immersed in low temperature solution were numerically calculated. It was suggested that ultrasound at high frequency is effective to enlarge supercooled region.
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2010 14th International Heat Transfer Conference
August 8–13, 2010
Washington, DC, USA
Conference Sponsors:
- Heat Transfer Division
ISBN:
978-0-7918-4936-1
PROCEEDINGS PAPER
Effects of Ultrasonic Irradiation on Ice Formation in Biological Tissue Available to Purchase
Yukio Tada,
Yukio Tada
Kanazawa University, Kanazawa, Ishikawa, Japan
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Yoshiaki Satou,
Yoshiaki Satou
Kanazawa University, Kanazawa, Ishikawa, Japan
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Makoto Kurokawa,
Makoto Kurokawa
Asahi Glass Co. Ltd., Ichihara, Chiba, Japan
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Akira Takimoto,
Akira Takimoto
Kanazawa University, Kanazawa, Ishikawa, Japan
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Hajime Onishi
Hajime Onishi
Kanazawa University, Kanazawa, Ishikawa, Japan
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Yukio Tada
Kanazawa University, Kanazawa, Ishikawa, Japan
Yoshiaki Satou
Kanazawa University, Kanazawa, Ishikawa, Japan
Makoto Kurokawa
Asahi Glass Co. Ltd., Ichihara, Chiba, Japan
Akira Takimoto
Kanazawa University, Kanazawa, Ishikawa, Japan
Hajime Onishi
Kanazawa University, Kanazawa, Ishikawa, Japan
Paper No:
IHTC14-22803, pp. 75-81; 7 pages
Published Online:
March 1, 2011
Citation
Tada, Y, Satou, Y, Kurokawa, M, Takimoto, A, & Onishi, H. "Effects of Ultrasonic Irradiation on Ice Formation in Biological Tissue." Proceedings of the 2010 14th International Heat Transfer Conference. 2010 14th International Heat Transfer Conference, Volume 1. Washington, DC, USA. August 8–13, 2010. pp. 75-81. ASME. https://doi.org/10.1115/IHTC14-22803
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