Nanoinjection lance arrays have been used to inject propidium iodide, a dye, into living cells. The lance arrays consist of approximately four million needle-like structures on a 2 × 2 cm silicon chip, fabricated using standard methods for silicon wafers. A culture of HeLa cancer cells, commonly used in genetic research, has been employed for testing the nanoinjection process. Propidium iodide at several concentrations has been used as the dye for cell injection. The dye binds to nucleic acids after injection, and does not fluoresce when not bound, allowing accurate flow cytometry measurement of successful injections. Over 200 wells were tested to gather reliable testing data, consisting of negative controls (no treatment), positive controls (dye added with no injection), and samples (dye added and injection performed). Tests were performed for dye concentrations of 20, 40, 60, and 80 μL per 1 mL of cell media solution, for two different lance array geometries. The multi-cell nanoinjection process presented in this paper has proven to produce positive dye uptake results in injected cells while maintaining high cell viability, with an average uptake rate of about 52% for cells injected with the highest concentration of dye. Reviewing all data, the average sample success rate is 8 times higher than the rate of dye uptake in the positive controls. Cell viability in these tests is on average 96.2%. The process may be used in a variety of research areas.
Skip Nav Destination
ASME 2013 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference
August 4–7, 2013
Portland, Oregon, USA
Conference Sponsors:
- Design Engineering Division
- Computers and Information in Engineering Division
ISBN:
978-0-7918-5584-3
PROCEEDINGS PAPER
Injections of Propidium Iodide Into HeLa Culture Cells Using a Nanoinjection Lance Array
Zachary K. Lindstrom,
Zachary K. Lindstrom
Brigham Young University, Provo, UT
Search for other works by this author on:
Steven J. Brewer,
Steven J. Brewer
Brigham Young University, Provo, UT
Search for other works by this author on:
Melanie A. Easter,
Melanie A. Easter
Brigham Young University, Provo, UT
Search for other works by this author on:
Brian D. Jensen,
Brian D. Jensen
Brigham Young University, Provo, UT
Search for other works by this author on:
Sandra H. Burnett
Sandra H. Burnett
Brigham Young University, Provo, UT
Search for other works by this author on:
Zachary K. Lindstrom
Brigham Young University, Provo, UT
Steven J. Brewer
Brigham Young University, Provo, UT
Melanie A. Easter
Brigham Young University, Provo, UT
Brian D. Jensen
Brigham Young University, Provo, UT
Sandra H. Burnett
Brigham Young University, Provo, UT
Paper No:
DETC2013-13281, V001T09A004; 6 pages
Published Online:
February 12, 2014
Citation
Lindstrom, ZK, Brewer, SJ, Easter, MA, Jensen, BD, & Burnett, SH. "Injections of Propidium Iodide Into HeLa Culture Cells Using a Nanoinjection Lance Array." Proceedings of the ASME 2013 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. Volume 1: 15th International Conference on Advanced Vehicle Technologies; 10th International Conference on Design Education; 7th International Conference on Micro- and Nanosystems. Portland, Oregon, USA. August 4–7, 2013. V001T09A004. ASME. https://doi.org/10.1115/DETC2013-13281
Download citation file:
10
Views
Related Proceedings Papers
Related Articles
A Device for the Automated Loading and Detection of Brachytherapy Elements Using Nonmechanical Methods for use in Prostate Cancer Treatment
J. Med. Devices (June,2012)
A Portable Aspiration-Assisted Device for End-Cut Prostate Biopsy of Large Tissue Sample
J. Med. Devices (January,0001)
Related Chapters
Colorectal Cancer Prognosis in Gene Expression Data
Intelligent Engineering Systems through Artificial Neural Networks Volume 18
Introduction
Thermal Management of Microelectronic Equipment, Second Edition
Antilock-Braking System Using Fuzzy Logic
International Conference on Mechanical and Electrical Technology, 3rd, (ICMET-China 2011), Volumes 1–3