There is considerable interest in measuring, with nanoscale spatial resolution, the physical properties of lipid membranes because of their role in the physiology of living systems. Due to its ability to nondestructively image surfaces in solution, tapping mode atomic force microscopy (TMAFM) has proven to be a useful technique for imaging lipid membranes. However, further information concerning the mechanical properties of surfaces is contained within the time-resolved tip/sample force interactions. The tapping forces can be recovered by taking the second derivative of the cantilever deflection signal and scaling by the effective mass of the cantilever; this technique is referred to as scanning probe acceleration microscopy. Herein, we describe how the maximum and minimum tapping forces change with surface mechanical properties. Furthermore, we demonstrate how these changes can be used to measure mechanical changes in lipid membranes containing cholesterol.
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ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference
August 12–15, 2012
Chicago, Illinois, USA
Conference Sponsors:
- Design Engineering Division
- Computers and Information in Engineering Division
ISBN:
978-0-7918-4504-2
PROCEEDINGS PAPER
Correlation of Atomic Force Microscopy Tapping Forces to Mechanical Properties of Lipid Membranes
Nicole Shamitko-Klingensmith,
Nicole Shamitko-Klingensmith
West Virginia University, Morgantown, WV
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Kelley M. Wambaugh,
Kelley M. Wambaugh
West Virginia University, Morgantown, WV
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Kathleen A. Burke,
Kathleen A. Burke
West Virginia University, Morgantown, WV
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George J. Magnone,
George J. Magnone
West Virginia University, Morgantown, WV
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Justin Legleiter
Justin Legleiter
West Virginia University, Morgantown, WV
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Nicole Shamitko-Klingensmith
West Virginia University, Morgantown, WV
Kelley M. Wambaugh
West Virginia University, Morgantown, WV
Kathleen A. Burke
West Virginia University, Morgantown, WV
George J. Magnone
West Virginia University, Morgantown, WV
Justin Legleiter
West Virginia University, Morgantown, WV
Paper No:
DETC2012-70233, pp. 303-310; 8 pages
Published Online:
September 9, 2013
Citation
Shamitko-Klingensmith, N, Wambaugh, KM, Burke, KA, Magnone, GJ, & Legleiter, J. "Correlation of Atomic Force Microscopy Tapping Forces to Mechanical Properties of Lipid Membranes." Proceedings of the ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. Volume 5: 6th International Conference on Micro- and Nanosystems; 17th Design for Manufacturing and the Life Cycle Conference. Chicago, Illinois, USA. August 12–15, 2012. pp. 303-310. ASME. https://doi.org/10.1115/DETC2012-70233
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