IgE (Immunoglobulin E) in the serum of an asthma patient is a useful index for allergy diseases. In this study, a nanostructured biosensor having uniformly deposited gold nanoparticles (GNPs) as the sensing electrode was used for fast and low serum consuming detection of the IgE in allergy patients’ serum. To enhance the charge transferring efficiency of the biosensor, silver nanoparticles (SNPs) were deposited on the GNP layer. The group 2 allergen, Der p2, was used as the probe to detect IgE. To ensure the specificity of detection, the affinity purified goat anti-Human IgE antibody was further immobilized to the IgE. After immobilizing the anti-IgE on the sensor, the electrochemical impedance spectroscopy (EIS) analysis was implemented to examine the concentration of the target IgE in terms of a Nyquist plot. Blood serum samples with known allergy levels detected by the commercially available ImmunoCAP were used for the verification of the sensor. It is observed that the difference of the charge transfer resistance (ΔRet) between the Der p2 immobilized electrode and the anti-IgE bonded electrode for each individual serum sample closely correlates to its ImmunoCAP class. The blood serum detection results indicate that the presented nanostructured biosensor is able to detect a patient’s allergy level with low sample consumption, short sample preparation time, and quick processing.
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ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference
August 12–15, 2012
Chicago, Illinois, USA
Conference Sponsors:
- Design Engineering Division
- Computers and Information in Engineering Division
ISBN:
978-0-7918-4504-2
PROCEEDINGS PAPER
In-Vitro Allergy Detection Using a Silver Nanoparticle Modified Nanostructured Biosensor
Gou-Jen Wang,
Gou-Jen Wang
National Chung-Hsing University, Taichung, Taiwan
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Yi-Fen Liu,
Yi-Fen Liu
National Chung-Hsing University, Taichung, Taiwan
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Chia-Che Wu
Chia-Che Wu
National Chung-Hsing University, Taichung, Taiwan
Search for other works by this author on:
Gou-Jen Wang
National Chung-Hsing University, Taichung, Taiwan
Yi-Fen Liu
National Chung-Hsing University, Taichung, Taiwan
Chia-Che Wu
National Chung-Hsing University, Taichung, Taiwan
Paper No:
DETC2012-70299, pp. 3-9; 7 pages
Published Online:
September 9, 2013
Citation
Wang, G, Liu, Y, & Wu, C. "In-Vitro Allergy Detection Using a Silver Nanoparticle Modified Nanostructured Biosensor." Proceedings of the ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. Volume 5: 6th International Conference on Micro- and Nanosystems; 17th Design for Manufacturing and the Life Cycle Conference. Chicago, Illinois, USA. August 12–15, 2012. pp. 3-9. ASME. https://doi.org/10.1115/DETC2012-70299
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