In AFM-based single molecule force spectroscopy, it is assumed that the pulling angle is negligible and that the force applied to the molecule is equivalent to the force measured by the instrument. Although this assumption may hold for flexible, compact molecules, studies have shown that it may not be appropriate for fairly rigid molecules, where measured forces can be a fraction of the actual values experienced by the molecule. Because the pulling geometry can substantially influence the values measured by the AFM, we investigate a method to minimize the pulling angle prior to conducting a pulling experiment. The method presented herein uses small circular movements to locate the molecule’s substrate attachment site and reposition the cantilever. By using data gathered from a previous study, we were able to repeatedly align a molecule’s attachment sites via simulation of the program, thereby demonstrating the effectiveness of the alignment method.
Skip Nav Destination
ASME 2007 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference
September 4–7, 2007
Las Vegas, Nevada, USA
Conference Sponsors:
- Design Engineering Division and Computers and Information in Engineering Division
ISBN:
0-7918-4804-3
PROCEEDINGS PAPER
A Method to Correct Pulling Geometry Induced Errors in Single Molecule Force Spectroscopy Measurements
Piotr E. Marszalek,
Piotr E. Marszalek
Duke University, Durham, NC
Search for other works by this author on:
Daniel G. Cole,
Daniel G. Cole
University of Pittsburgh, Pittsburgh, PA
Search for other works by this author on:
Robert L. Clark
Robert L. Clark
Duke University, Durham, NC
Search for other works by this author on:
Monica Rivera
Duke University, Durham, NC
Changhong Ke
Duke University, Durham, NC
Piotr E. Marszalek
Duke University, Durham, NC
Daniel G. Cole
University of Pittsburgh, Pittsburgh, PA
Robert L. Clark
Duke University, Durham, NC
Paper No:
DETC2007-35430, pp. 801-807; 7 pages
Published Online:
May 20, 2009
Citation
Rivera, M, Ke, C, Marszalek, PE, Cole, DG, & Clark, RL. "A Method to Correct Pulling Geometry Induced Errors in Single Molecule Force Spectroscopy Measurements." Proceedings of the ASME 2007 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. Volume 3: 19th International Conference on Design Theory and Methodology; 1st International Conference on Micro- and Nanosystems; and 9th International Conference on Advanced Vehicle Tire Technologies, Parts A and B. Las Vegas, Nevada, USA. September 4–7, 2007. pp. 801-807. ASME. https://doi.org/10.1115/DETC2007-35430
Download citation file:
3
Views
0
Citations
Related Proceedings Papers
Related Articles
Utilizing Off-Resonance and Dual-Frequency Excitation to Distinguish Attractive and Repulsive Surface Forces in Atomic Force Microscopy
J. Comput. Nonlinear Dynam (July,2011)
Influence of Local Material Properties on the Nonlinear Dynamic Behavior of an Atomic Force Microscope Probe
J. Comput. Nonlinear Dynam (October,2011)
Related Chapters
A Study on the Application of the Vehicle's Intelligent Weighing System
International Conference on Information Technology and Computer Science, 3rd (ITCS 2011)
Key Technologies of Instrument Simulation in the Virtual Maintenance System
International Conference on Instrumentation, Measurement, Circuits and Systems (ICIMCS 2011)
A Method for Matching Two-Dimensional Contours
International Conference on Mechanical and Electrical Technology, 3rd, (ICMET-China 2011), Volumes 1–3