The effects of rolling condition on the flow instability characteristics of natural circulation (NC) in rod bundle channel were experimentally studied. A 3 × 3 rod bundle channel is used as the testing section. The experimental system pressure range is 0.2 to 0.6 MPa, and the range of inlet subcooling is 10 to 70 °C. The ranges of rolling motion amplitude and period are 10 ∼ 20° and 10 ∼ 30s, respectively. Two typical two-phase flow instabilities in rod bundle channel under rolling condition were found in experiments: (a) the trough-type oscillation caused by the vapor generation at the minimum point of flow fluctuation and (b) the compound oscillation formed by the superposition of the trough-type oscillation and DWOI. Experimental results show that the rolling motion can reduce the threshold heating power of trough-type oscillation and cause the occurrence of NC flow instability in advance. But the rolling motion cannot affect the dimensionless boundary of DWOI in rod bundle channel.