The friction characteristics of water in a sub-millimeter scale channel were investigated experimentally. The friction factors and the critical Reynolds number were measured using water flow through circular tubes with diameters of 0.5, 0.25 and 0.17 mm. The experimental results show that the measured friction factor for water agreed well with the conventional Poiseuille (λ = 64/Re) and Blasius (λ = 0.316 Re−0.25) equations in laminar and turbulent flow regime; the laminar-turbulent transition Reynolds number was approximately 2300 for diameter 0.5 mm. For diameter 0.25 mm, the friction factor evaluated by the form pressure drop also agreed well with the Poiseuille equation. For diameter 0.17 mm, the measured total friction factor was close to the Poiseuille prediction.

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