We report on a novel microfluidic strategy for building monodisperse asymmetric vesicles with customized composition, size, and interfacial properties at high-throughput. The microfluidic device encompasses a triangular post region and two flow-focusing regions. The major steps involved in the vesicle building process include: (1) forming highly uniform water emulsion templates in the inner-leaflet lipid solution, (2) replacing the inner-leaflet lipid solution with the outer-leaflet lipid solution, (3) creating water-in-oil-in-water double emulsions, and (4) extracting the excess outer-leaflet lipid solution from the double emulsions. Bilayer membrane asymmetry and unilamellarity are confirmed using a fluorescence quenching assay and quantitative measurements of fluorescent intensities. This method addresses many of the deficiencies found in existing technologies, and yields asymmetries as high as 95%. The asymmetric vesicles built using this strategy hold the potential to serve as model systems to investigate fundamental problems in membrane biology.
- Heat Transfer Division
- Fluids Engineering Division
Synthetic Asymmetric Vesicles Built Using Microfluidic Technology at High-Throughput
Lu, L, Schertzer, JW, & Chiarot, PR. "Synthetic Asymmetric Vesicles Built Using Microfluidic Technology at High-Throughput." Proceedings of the ASME 2015 13th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2015 International Technical Conference and Exhibition on Packaging and Integration of Electronic and Photonic Microsystems. ASME 2015 13th International Conference on Nanochannels, Microchannels, and Minichannels. San Francisco, California, USA. July 6–9, 2015. V001T03A006. ASME. https://doi.org/10.1115/ICNMM2015-48556
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