We propose a microfluidic droplet-based platform that accepts an unlimited number of sample plugs from a multi-well plate, performs splitting of these sample droplets into smaller daughter droplets and subsequent synchronization-free, reliable fusion of sample daughter droplets with multiple reagents simultaneously. This system consists of two components: 1) a custom autosampler which generates a linear array of sub-microliter plugs in a microcapillary from a multi-well plate and 2) A microfluidic chip with channels for sample plug introduction, reagent merging and droplet incubation. This novel system generates large arrays of heterogeneous droplets from hundreds to thousands of samples while concurrently screening these arrays against a large array of reagents. This high throughput system minimizes sample and reagent consumption and can be applied to a gamut of biological assays, ranging from SNP detection to forensic screening.
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ASME 2012 10th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2012 Heat Transfer Summer Conference and the ASME 2012 Fluids Engineering Division Summer Meeting
July 8–12, 2012
Rio Grande, Puerto Rico, USA
Conference Sponsors:
- Heat Transfer Division
- Fluids Engineering Division
ISBN:
978-0-7918-4479-3
PROCEEDINGS PAPER
Microfluidic Combinatorial Screening Platform Available to Purchase
Helena Zec,
Helena Zec
Johns Hopkins University, Baltimore, MD
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Tushar D. Rane,
Tushar D. Rane
Johns Hopkins University, Baltimore, MD
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Wen-Chy Chu,
Wen-Chy Chu
Pioneer Hi-Bred International Inc., Johnston, IA
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Tza-Huei Wang
Tza-Huei Wang
Johns Hopkins University, Baltimore, MD
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Helena Zec
Johns Hopkins University, Baltimore, MD
Tushar D. Rane
Johns Hopkins University, Baltimore, MD
Wen-Chy Chu
Pioneer Hi-Bred International Inc., Johnston, IA
Tza-Huei Wang
Johns Hopkins University, Baltimore, MD
Paper No:
ICNMM2012-73159, pp. 91-96; 6 pages
Published Online:
July 22, 2013
Citation
Zec, H, Rane, TD, Chu, W, & Wang, T. "Microfluidic Combinatorial Screening Platform." Proceedings of the ASME 2012 10th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2012 Heat Transfer Summer Conference and the ASME 2012 Fluids Engineering Division Summer Meeting. ASME 2012 10th International Conference on Nanochannels, Microchannels, and Minichannels. Rio Grande, Puerto Rico, USA. July 8–12, 2012. pp. 91-96. ASME. https://doi.org/10.1115/ICNMM2012-73159
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