White blood cell (WBC) sequestration in lung capillaries is a key step in the inflammatory response to lung infection. P-selectin and ICAM-1 have well-defined roles in WBC adhesion in venules but their role in pulmonary capillaries is still unclear. Here, a novel in vitro Micropipette Cell Adhesion Assay used P-selectin, ICAM-1 or BSA-coated capillary-sized glass micropipettes as an in vitro model of a lung capillary. WBC were aspirated into adhesion molecule-coated vessels of varying diameters. Cell velocities and activation times were determined under pressures representative of lung capillaries. WBC velocities in this assay were significantly lower on P-selectin than BSA and decreased with increasing P-selectin concentration. These results demonstrate that P-selectin at low density mediates WBC adhesion in the pulmonary capillary geometry. WBC can also become activated upon aspiration into micropipettes and under some circumstances can be seen to exhibit a cyclic migratory behavior. This work was supported by grant BES-0547165 from the National Science Foundation and by an award from the American Heart Association.
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ASME 2010 8th International Conference on Nanochannels, Microchannels, and Minichannels collocated with 3rd Joint US-European Fluids Engineering Summer Meeting
August 1–5, 2010
Montreal, Quebec, Canada
Conference Sponsors:
- Fluids Engineering Division
ISBN:
978-0-7918-5450-1
PROCEEDINGS PAPER
Neutrophil Motion, Adhesion and Activation in an In Vitro Micropipette Model of a Lung Capillary
David F. J. Tees,
David F. J. Tees
Ohio University, Athens, OH
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Prithu Sundd,
Prithu Sundd
La Jolla Institute of Allergy & Immunology, La Jolla, CA
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Young Eun Choi,
Young Eun Choi
Ohio University, Athens, OH
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Douglas J. Goetz,
Douglas J. Goetz
Ohio University, Athens, OH
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Steven Rogers
Steven Rogers
Ohio University, Athens, OH
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David F. J. Tees
Ohio University, Athens, OH
Prithu Sundd
La Jolla Institute of Allergy & Immunology, La Jolla, CA
Young Eun Choi
Ohio University, Athens, OH
Douglas J. Goetz
Ohio University, Athens, OH
Anand Pai
Duke University, Durham, NC
Steven Rogers
Ohio University, Athens, OH
Paper No:
FEDSM-ICNMM2010-30177, pp. 9-19; 11 pages
Published Online:
March 1, 2011
Citation
Tees, DFJ, Sundd, P, Choi, YE, Goetz, DJ, Pai, A, & Rogers, S. "Neutrophil Motion, Adhesion and Activation in an In Vitro Micropipette Model of a Lung Capillary." Proceedings of the ASME 2010 8th International Conference on Nanochannels, Microchannels, and Minichannels collocated with 3rd Joint US-European Fluids Engineering Summer Meeting. ASME 2010 8th International Conference on Nanochannels, Microchannels, and Minichannels: Parts A and B. Montreal, Quebec, Canada. August 1–5, 2010. pp. 9-19. ASME. https://doi.org/10.1115/FEDSM-ICNMM2010-30177
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