A simple, low-cost technique for high throughput single-cell analysis, Microscale Oil-Covered Cell Array (MOCCA), is presented in this paper. Corresponding to recent research on single cell analysis, simple devices for isolated cell chambers are urgently needed and long sought-after. Instead of using microfabricated solid structures to capture cells, MOCCA isolates cells in discrete aqueous droplets that are separated by oil on the patterned hydrophilic areas on a relatively more hydrophobic flat substrate. In our pioneer study, we created an array of 700-picoliter droplets. The randomly seeded E. coli cell number in each discrete droplet approaches single-cell levels. The total time needed for MOCCA fabrication was no more than 10 minutes. Compared to traditional micro-fabrication techniques, MOCCA dramatically lowers the cost and enhances the efficiency for the fabrication procedure, while producing a microscale array as in those made using traditional methods.
- Nanotechnology Institute
Microscale Oil-Covered Cell Array (MOCCA): A Droplet Array for High-Content Single-Cell Analysis and Imaging
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Lin, L, Chao, S, & Meldrum, DR. "Microscale Oil-Covered Cell Array (MOCCA): A Droplet Array for High-Content Single-Cell Analysis and Imaging." Proceedings of the ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels. ASME 2009 7th International Conference on Nanochannels, Microchannels and Minichannels. Pohang, South Korea. June 22–24, 2009. pp. 669-672. ASME. https://doi.org/10.1115/ICNMM2009-82283
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