Technological needs of the recent times require the improvement in micro-scale devices that manipulate the bioparticles like cells, bacteria, viruses, DNA, proteins, etc. Such devices have diverse and widespread applications in biomedical, drug delivery and diagnostics for separating, trapping, sorting and mixing of particles. Dielectrophoresis (DEP) is one of the techniques used for manipulating the particles in a nonuniform electric field. In the present study, fabrication and characterization of microfluidic device for DEP is analyzed and experimented. An overview of fabrication techniques which can be used for making of DEP device is provided with experimental details. DEP microfluidic device is fabricated by preparing channels and microelectrodes on PDMS and glass materials respectively. Oxygen plasma treatment has been used for bonding the PDMS channel and micro-electrode patterned glass substrate. Further experiments are conducted to demonstrate the DEP principle with polystyrene microbeads. The movement of microbeads towards the high electric field strengths at 12Vpp and 10 MHz frequency is observed. Characterizing equipments like ellipsometer, profilometer, scanning electron microscopy, contact angle measurement systems were used for measuring oxide layer thickness, width and depth of the channels, surface characteristics etc., during fabrication.
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ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels
June 22–24, 2009
Pohang, South Korea
Conference Sponsors:
- Nanotechnology Institute
ISBN:
978-0-7918-4349-9
PROCEEDINGS PAPER
Fabrication of Dielectrophoretic Microfluidic Device
G. Naga Siva Kumar,
G. Naga Siva Kumar
University of Alberta, Edmonton, AB, Canada
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Sushanta K. Mitra,
Sushanta K. Mitra
University of Alberta, Edmonton, AB, Canada
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V. Ramgopal Rao
V. Ramgopal Rao
Indian Institute of Technology - Bombay, Mumbai, India
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G. Naga Siva Kumar
University of Alberta, Edmonton, AB, Canada
Sushanta K. Mitra
University of Alberta, Edmonton, AB, Canada
V. Ramgopal Rao
Indian Institute of Technology - Bombay, Mumbai, India
Paper No:
ICNMM2009-82170, pp. 113-119; 7 pages
Published Online:
September 21, 2010
Citation
Kumar, GNS, Mitra, SK, & Rao, VR. "Fabrication of Dielectrophoretic Microfluidic Device." Proceedings of the ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels. ASME 2009 7th International Conference on Nanochannels, Microchannels and Minichannels. Pohang, South Korea. June 22–24, 2009. pp. 113-119. ASME. https://doi.org/10.1115/ICNMM2009-82170
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