Recruitment rate measurements of micro-scale particles, such as cells or microbeads, to biofunctional surfaces is difficult because factors such as uneven ligand distributions, particle collisions, variable particle fluxes, and molecular-scale surface separation distances that combine to obfuscate the ability to link the observed particle behavior with the governing nanoscale biophysics. We report the development of a hydrodynamically-conditioned micropattern catch strip assay to measure microparticle and cellular recruitment kinetics. The assay exploited patterning within microfluidic channels and the mechanostability of selectin bonds to create reaction geometries that confined a microbead flux to within 200 nm of the surface under flow conditions. Systematic control of capillary action enabled the creation of homogenous or gradient ligand distributions. The method enabled the measurement of particle recruitment rates (keff, s−1) that were primarily determined by the interaction of the biomolecular pair being investigated. The method may be well suited for analysis of reaction rates between surface-bound molecules in the presence of convective flow patterns.
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ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels
June 23–25, 2008
Darmstadt, Germany
Conference Sponsors:
- Nanotechnology Institute
ISBN:
0-7918-4834-5
PROCEEDINGS PAPER
A Micropatterned Surface for the Assessment of Relative Two-Dimensional Molecular Selectin Kinetics in Flow Available to Purchase
Michael B. Lawrence,
Michael B. Lawrence
University of Virginia, Charlottesville, VA
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Brian J. Schmidt
Brian J. Schmidt
University of Virginia, Charlottesville, VA
Search for other works by this author on:
Michael B. Lawrence
University of Virginia, Charlottesville, VA
Brian J. Schmidt
University of Virginia, Charlottesville, VA
Paper No:
ICNMM2008-62046, pp. 1771-1777; 7 pages
Published Online:
June 11, 2009
Citation
Lawrence, MB, & Schmidt, BJ. "A Micropatterned Surface for the Assessment of Relative Two-Dimensional Molecular Selectin Kinetics in Flow." Proceedings of the ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels. ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels. Darmstadt, Germany. June 23–25, 2008. pp. 1771-1777. ASME. https://doi.org/10.1115/ICNMM2008-62046
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