We have developed a method to detect non-fluorescent analytes on standard microfluidic chip platforms equipped with fluorescence detection. We leverage isotachophoresis (ITP) to electrophoretically segregate both analytes and fluorescent species termed mobility markers into distinct zones. The fluorescent marker zones bound analyte zones, so that gaps in the fluorescent signal indicate the presence and concentration of analytes. We here demonstrate separation and indirect detection of amino acids, serine and phenylalanine and organic acids, acetic acid and phenylpropionic acid (∼10 μM) using this technique. We also present an indirect detection of the environmental toxin phenol [1][2] (∼10 μM) using two mobility markers. We show preliminary numerical simulation results that provide useful guidelines in design and optimization of our indirect detection assay.
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ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels
June 23–25, 2008
Darmstadt, Germany
Conference Sponsors:
- Nanotechnology Institute
ISBN:
0-7918-4834-5
PROCEEDINGS PAPER
Indirect Fluorescence Detection of Non Fluorescent Analytes Using Isotachophoretic Mobility Markers
Tarun K. Khurana,
Tarun K. Khurana
Stanford University, Stanford, CA
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Moran Bercovici,
Moran Bercovici
Stanford University, Stanford, CA
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Juan G. Santiago
Juan G. Santiago
Stanford University, Stanford, CA
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Tarun K. Khurana
Stanford University, Stanford, CA
Moran Bercovici
Stanford University, Stanford, CA
Juan G. Santiago
Stanford University, Stanford, CA
Paper No:
ICNMM2008-62027, pp. 1701-1706; 6 pages
Published Online:
June 11, 2009
Citation
Khurana, TK, Bercovici, M, & Santiago, JG. "Indirect Fluorescence Detection of Non Fluorescent Analytes Using Isotachophoretic Mobility Markers." Proceedings of the ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels. ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels. Darmstadt, Germany. June 23–25, 2008. pp. 1701-1706. ASME. https://doi.org/10.1115/ICNMM2008-62027
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