Micro fluid segments attract increasing interest due to the possibility of easy generation, manipulation and use for diagnostics, chemical analytics, synthesis, cell cultivation and chemical as well as biological screenings. The addressing of chemical composition is a very important precondition for the application of micro fluid segments in highly parallelized operations. Here, we report the addressing of larger concentration spaces by use of a double-twin injector made in micro lithographic glass/glass technology. Two-dimensional concentration distributions can be achieved by varying flow rate ratios during the formation of fluid segments. Two different dyes (Orange G and Crystal violet) have been used to generate the two dimensional concentration space. The composition of dye containing segments is monitored by in-line flow-through micro photometry.

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