A microfluidic chip-based solid phase extraction method for isolation of nucleic acids is demonstrated. The chip was fabricated in a cyclic polyolefin by hot embossing with a master. The solid phase was made by in-situ UV polymerization of a monolithic column impregnated with silica particles, and separation was achieved due to irreversible binding of the nucleic acids to the silica particles in the monolith. The porous monolithic column was formed within the channels of the device by photoinitiated polymerization of a mixture of methacrylate and dimethacrylate monomers, UV sensitive free radical initiator and porogenic solvent. The channel surface was photografted with a thin interlayer polymer prior to preparation of the monolith in the channel. The grafted layer covalently attached the monolith and prevented the formation of voids between the monolith and the channel surface. The solid-phase prepared by this method allowed for successful extraction and elution of nucleic acids.
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ASME 3rd International Conference on Microchannels and Minichannels
June 13–15, 2005
Toronto, Ontario, Canada
Conference Sponsors:
- Nanotechnology Institute
ISBN:
0-7918-4185-5
PROCEEDINGS PAPER
Fabrication of Polymeric Microfluidic Device for On-Chip Isolation of Nucleic Acids
Arpita Bhattacharyya,
Arpita Bhattacharyya
Boston University, Boston, MA
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Catherine Klapperich
Catherine Klapperich
Boston University, Boston, MA
Search for other works by this author on:
Arpita Bhattacharyya
Boston University, Boston, MA
Catherine Klapperich
Boston University, Boston, MA
Paper No:
ICMM2005-75200, pp. 551-556; 6 pages
Published Online:
November 11, 2008
Citation
Bhattacharyya, A, & Klapperich, C. "Fabrication of Polymeric Microfluidic Device for On-Chip Isolation of Nucleic Acids." Proceedings of the ASME 3rd International Conference on Microchannels and Minichannels. ASME 3rd International Conference on Microchannels and Minichannels, Part B cont’d. Toronto, Ontario, Canada. June 13–15, 2005. pp. 551-556. ASME. https://doi.org/10.1115/ICMM2005-75200
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