We report a method of producing minute micro-droplets through nanoscale molecular self-assembly from a given liquid volume without external input. By the branching of hydrophobic restrictions, the drop is forced to separate while translating on a flat surface. Symmetry in the drop front wetted perimeter conduces to equal division of the drop. We show that at least 3 divisions can be performed sequentially on a 1.5 microliter drop to give minute droplets of approximately equal volumes. A division of carrier liquid volume by 1/23 enables multiple analyses on many separate stations in one microfluidic application.

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