Energy addition using a hot surface probe is required for reliable ignition of aircraft compression ignition engines running on fuel variations and at altitude conditions. Thus, durability of the hot surface probe is crucial for application in these engines. Thermo-mechanical stress is one of the key parameters that determine durability, which requires an accurate prediction of the transient temperature field based on well-defined boundary conditions representing the dynamic and complex fluid flow inside engines. To meet this requirement, the present study focuses on transient thermo-mechanical stress analysis using a sequentially coupled CFD-FEA approach to understand transient thermo-mechanical responses of the hot surface probe. A 3D transient reacting flow simulation was conducted first using CONVERGE software, the results of which were exported to map thermal and pressure boundary conditions onto a structural finite element mesh. Transient thermo-mechanical stress analysis was performed sequentially using ABAQUS software utilizing the mapped boundary conditions. The results such as transient temperature history, resultant thermo-mechanical stress, displacement, potential failure modes, etc. were critically reviewed, which can provide helpful information for further design improvement.