This work evaluated the genotoxic potential of the soluble organic material (SOM) extracted from the particulate matter (PM) emitted by an automotive diesel engine. The engine was modified to operate with a home-made multipoint-port injection system to substitute 10% of ultra-low-sulfur diesel (ULSD) fuel in energy basis by hydrous ethanol (h-Et) or n-butanol (n-Bu) injected into the manifold during the intake stroke. A low engine load mode named M4 (43 Nm at 2410 min−1) and a medium-load mode M2 (95 Nm at 2410 min−1) were selected from the vehicle homologation cycle. PM was collected with a stainless steel filter located 1.5 m downstream the exhaust manifold. The SOM of the PM was extracted to evaluate the genotoxic activity on human lymphocytes using the Comet assay. Results indicated that independently of the mode, the SOM coming from alcohols led more genotoxicity than ULSD, following the order h-Et > n-Bu > ULSD. The low engine load operation exhibited much more DNA damage than mode M2, especially the PM produced by hydrous ethanol port-injection. Although further research is still necessary, these findings suggest that the biology activity of the SOM coming from alcohols PM could be a barrier for the implementation of alcohol port-injection technology.
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ASME 2016 Internal Combustion Engine Division Fall Technical Conference
October 9–12, 2016
Greenville, South Carolina, USA
Conference Sponsors:
- Internal Combustion Engine Division
ISBN:
978-0-7918-5050-3
PROCEEDINGS PAPER
Genotoxicity of Diesel Particulate Matter Emitted by Port-Injection of Hydrous Ethanol and N-Butanol Available to Purchase
Marlon Cadrazco,
Marlon Cadrazco
Universidad de Antioquia, Medellín, Colombia
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John R. Agudelo,
John R. Agudelo
Universidad de Antioquia, Medellín, Colombia
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Luz Y. Orozco,
Luz Y. Orozco
Universidad de Antioquia, Medellín, Colombia
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Verónica Estrada
Verónica Estrada
Universidad de Antioquia, Medellín, Colombia
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Marlon Cadrazco
Universidad de Antioquia, Medellín, Colombia
John R. Agudelo
Universidad de Antioquia, Medellín, Colombia
Luz Y. Orozco
Universidad de Antioquia, Medellín, Colombia
Verónica Estrada
Universidad de Antioquia, Medellín, Colombia
Paper No:
ICEF2016-9409, V001T02A012; 7 pages
Published Online:
December 1, 2016
Citation
Cadrazco, M, Agudelo, JR, Orozco, LY, & Estrada, V. "Genotoxicity of Diesel Particulate Matter Emitted by Port-Injection of Hydrous Ethanol and N-Butanol." Proceedings of the ASME 2016 Internal Combustion Engine Division Fall Technical Conference. ASME 2016 Internal Combustion Engine Division Fall Technical Conference. Greenville, South Carolina, USA. October 9–12, 2016. V001T02A012. ASME. https://doi.org/10.1115/ICEF2016-9409
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