Polymerase-chain-Reaction (PCR) is a thermal cycling (repeated heating and cooling of PCR solution) process for DNA amplification. PCR is the key ingredient in many biomedical applications. One key feature for the success of the PCR is to control the temperature of the solution precisely at the desired temperature levels required for the PCR in a cyclic manner. Microfluidics offers a great advantage over conventional techniques since minute amounts of PCR solution can be heated and cooled with a high rate in a controlled manner. In this study, a microfluidic platform has been proposed for continuous-flow PCR. The microfluidic device consists of a spiral channel on a glass wafer with integrated chromium microheaters. Sub-micron thick microheaters are deposited beneath the micro-channels to facilitate localized heating. The microfluidic device is modeled using COMSOL Multiphysics®. The fabrication procedure of the device is also discussed and future research directions are addressed. With its compact design, the proposed system can easily be coupled with an integrated microfluidic device to be used in biomedical applications.
- Heat Transfer Division
Spiral Microfluidics Device for Continuous Flow PCR
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Salemmilani, R, & Cetin, B. "Spiral Microfluidics Device for Continuous Flow PCR." Proceedings of the ASME 2013 Heat Transfer Summer Conference collocated with the ASME 2013 7th International Conference on Energy Sustainability and the ASME 2013 11th International Conference on Fuel Cell Science, Engineering and Technology. Volume 2: Heat Transfer Enhancement for Practical Applications; Heat and Mass Transfer in Fire and Combustion; Heat Transfer in Multiphase Systems; Heat and Mass Transfer in Biotechnology. Minneapolis, Minnesota, USA. July 14–19, 2013. V002T11A001. ASME. https://doi.org/10.1115/HT2013-17305
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