In the present study, cultured normal rat microvessel endothelial cells are first used to explore the feasibility of studying the cell metabolic activities by detecting autofluorescence intensities. A mercury lamp is used to induce autofluorescence emissions in cells. Under an excitation wavelength of 360nm, the emissions peak at 470 nm corresponds with the autofluorescence signature of NAD(P)H, the concentration of which has been linked to the metabolic rate of the cell. A relation between the emission intensity and the NAD(P)H concentration is established. Based upon the results and observations of the endothelial cells, a protocol is developed for studying the autofluorescence of other cultured cells. It has been long known that cancer cells divide much more rapidly than normal cells depending on the degree of the tumor malignancy. The differences in metabolic rates of normal and cancerous breast cells are explored as they relate to autofluorescence emissions at different wavelengths and intensities. The comparison results can be used as a guideline to determine whether cells are cancerous. At a tissue level, laser excitation is probably needed to obtain measurable autofluorescence signals. The potential of autofluorescence measurement as a breast cancer diagnostic tool is discussed.
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ASME 2003 Heat Transfer Summer Conference
July 21–23, 2003
Las Vegas, Nevada, USA
Conference Sponsors:
- Heat Transfer Division
ISBN:
0-7918-3694-0
PROCEEDINGS PAPER
Study of Cell Metabolic Activities Using Fluorescence Microscopy Available to Purchase
Dawn M. Sabados,
Dawn M. Sabados
Purdue University, West Lafayette, IN
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Lisa X. Xu,
Lisa X. Xu
Purdue University, West Lafayette, IN
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Jay P. Gore
Jay P. Gore
Purdue University, West Lafayette, IN
Search for other works by this author on:
Dawn M. Sabados
Purdue University, West Lafayette, IN
Lisa X. Xu
Purdue University, West Lafayette, IN
Jay P. Gore
Purdue University, West Lafayette, IN
Paper No:
HT2003-47186, pp. 781-783; 3 pages
Published Online:
December 17, 2008
Citation
Sabados, DM, Xu, LX, & Gore, JP. "Study of Cell Metabolic Activities Using Fluorescence Microscopy." Proceedings of the ASME 2003 Heat Transfer Summer Conference. Heat Transfer: Volume 2. Las Vegas, Nevada, USA. July 21–23, 2003. pp. 781-783. ASME. https://doi.org/10.1115/HT2003-47186
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