This study carries out parametric investigations on aerodynamic loss of various types of LP turbine airfoils characterized with different flow deceleration rates (DR) on their suction surfaces under the realistic flow conditions such as wake inflow and freestream turbulence. The Reynolds number examined in this study ranges from 57,000 to 170,000. As for the freestream turbulence, two levels of the turbulence are used, i.e., about 1.2% and 3.5%. Stagnation pressure distributions downstream of each of the airfoil cascades are measured by use of a Pitot tube, while steady-state and unsteady boundary-layers are measured over the rear part of suction surface and pressure side near the trailing edge using a single hot-wire probe. The measured boundary-layer data are used to estimate the cascade loss along with RANS (Reynolds-Averaged Navier-Stokes) simulations by taking advantage of the momentum-theory based Denton’s method. First, relationships between the cascade loss for each flow condition and DR are examined. The estimated loss values are then compared with the measured cascade loss to check the validity of the loss estimation method, which is a derivative of Denton’s method, under the realistic flow conditions.