The compressibility of flow field has an important effect on flow stability. However, when the compressibility is considered, the effect of Mach number is often considered while the effect of heat transfer is always neglected in the existing flow stability studies. Linear stability analysis tools based on compressible Orr-Sommerfeld (O-S) equations and linearized Navier-Stokes equations in cylindrical coordinate system are established in this paper. These equations are numerically solved by using Chebyshev spectral collocation method and pseudo-modes are eliminated. Linear stability analysis of pipe flow with heat transfer whose average flow field is obtained by CFD simulation is carried out. The results show that for spatial modes, the heating effect of the wall makes pipe flow more unstable, while cooling effect of the wall makes pipe flow more stable. For global modes of pipe flow, the frequency of global mode decreases when the wall cools the flow and the decrease of mean temperature of pipe flow leads to the improvement of global mode stability.

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