Bead-based immunosensing has been growing as a promising technology in the point-of-care diagnostics because of great flexibility. For dilute samples, functionalized particles can be used to collect dispersed analytes and act as carriers for particle manipulation. To carry out rapid and selective diagnosis, a bead-based optoelectrokinetic immunosensing technique was developed herein to detect biomarkers, lipocalin 1 (LCN1) and TNF-α, for diabetic retinopathy (DR). The measurement was made in a sample droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam simultaneously applying on the microchip, the immunocomplexes in the droplet were further concentrated within the region of irradiation to enhance the fluorescent signal. The optoelectrokinetic platform, termed rapid electrokinetic patterning (REP), is excellent in dynamic and programmable particle manipulation. Therefore, the detection could be complete in roughly 10 s. With an appropriate frequency modulation, the two DR biomarkers were detected at a time. The limit of detection (LOD) of the REP-enabled measurement reached as low as 100 pg/mL. The combined use of bead-based immunoassays and the optoelectrokinetic platform therefore provides an insightful measure to the early diagnosis of diseases.
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ASME 2017 Fluids Engineering Division Summer Meeting
July 30–August 3, 2017
Waikoloa, Hawaii, USA
Conference Sponsors:
- Fluids Engineering Division
ISBN:
978-0-7918-5805-9
PROCEEDINGS PAPER
Development of a Bead-Based Optoelectrokinetic Immunosensing Technique for Detection of Low-Abundance Analytes Available to Purchase
Han-Sheng Chuang,
Han-Sheng Chuang
National Cheng Kung University, Tainan, Taiwan
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Hsiao-Neng Lin,
Hsiao-Neng Lin
National Cheng Kung University, Tainan, Taiwan
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Hu-Yao Ku
Hu-Yao Ku
National Cheng Kung University, Tainan, Taiwan
Search for other works by this author on:
Han-Sheng Chuang
National Cheng Kung University, Tainan, Taiwan
Hsiao-Neng Lin
National Cheng Kung University, Tainan, Taiwan
Hu-Yao Ku
National Cheng Kung University, Tainan, Taiwan
Paper No:
FEDSM2017-69146, V01BT10A006; 5 pages
Published Online:
October 24, 2017
Citation
Chuang, H, Lin, H, & Ku, H. "Development of a Bead-Based Optoelectrokinetic Immunosensing Technique for Detection of Low-Abundance Analytes." Proceedings of the ASME 2017 Fluids Engineering Division Summer Meeting. Volume 1B, Symposia: Fluid Measurement and Instrumentation; Fluid Dynamics of Wind Energy; Renewable and Sustainable Energy Conversion; Energy and Process Engineering; Microfluidics and Nanofluidics; Development and Applications in Computational Fluid Dynamics; DNS/LES and Hybrid RANS/LES Methods. Waikoloa, Hawaii, USA. July 30–August 3, 2017. V01BT10A006. ASME. https://doi.org/10.1115/FEDSM2017-69146
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