Digital in-line holography has been applied extensively to particle field measurement, where the particles can be the tracer particles for flow measurements, droplets in spray diagnostics, or aquatic microorganisms. The particle detection algorithm plays a key role in accurate detection of particle 3D locations and sizes. A hybrid method has recently been proposed for particle field measurement in digital in-line holography. It features automatic determination of the threshold for segmentation of the particle field and the capability to measure particles of arbitrary shapes. Using the hybrid method, the particle 3D location, size and shape information can be extracted. The uncertainty of the hybrid method is characterized using both synthetic and experimental holograms at different particle field conditions. The depth measurement uncertainty is within 2 times the particle diameter for a wide range of particle field conditions.

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