Microfluidic paper-based analytical devices (μPADs) are cost-effective point-of-care diagnostic devices. μPADs consist of porous filter paper patterned with hydrophobic solid ink barriers to create flow channels. Because a liquid sample flows through the paper channel driven by capillary force, the resultant flow is usually slow. To overcome this limitation, a hollow channel can be added to a μPAD to increase the flow speed significantly. The liquid flow through the hollow channel is known to be driven by a pressure difference between the inlet and outlet of the device. Accordingly, theoretical models have been proposed to understand and predict flow characteristics of μPADs with hollow channels. The goal of this study is to experimentally characterize liquid flow through μPADs having a hollow channel, by investigating relationships among the travel distance of the liquid front through the μPADs, the applied pressure difference, and the dimension of the hollow channel. Thus, the outcome of this study would contribute to validating the theoretical models and enable better control of liquid sample flow in μPADs with hollow channels.