Size-Exclusion Surface Plasmon Resonance Sensing With a Microslit Array Chip

In this study, we propose a novel surface plasmon resonance (SPR) chip on which the microslit array was fabricated. The microslit excludes micrometer-size objects that are larger than its slit size from sensing field, so that it acts as a filter. In order to confirm the filtereffect, we demonstrated the sensing of microparticles of different diameters using the SPR chip. As the demonstration of the biotechnology application, we performed the discrimination of aggregation of bio-molecules using SGNP (Sugar chain imobilized Gold Nano Particle) as a model sample.