Effect of multiple operation conditions on behaviors of H2 production from organic substrate by photo-fermentation in immobilized-cells packed bed using Rhodoseudomonas palustris CQK 01 were investigated in a continuous culture. It revealed that in limited light intensity or below 590 nm of light wavelength the H2 production rate of the immobilized-cells bioreactor increased with increase of value of the experimental parameters. And the optimal operation factor of inlet concentration of substrate and inlet temperature of liquid for H2 production was 50 mmol/L and 30 °C, respectively. However, when these parameters value in the experiment became suppressive conditions the H2 production rate would went down with parameter ascending.

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