Monophasic action potentials (MAPs) have long been used as a means to study the focal electrical activity of the myocardium. [1, 2] Upon the application of adequate contact force, the signals provide important insights into focal depolarization and repolarization, activation timing, and focal arrhythmic behaviors. [3–6]

Within our laboratory we have developed an isolated physiologic, four-chamber working, large mammalian heart model (the Visible Heart® methodology) to study cardiac devices and their interactions with the myocardium. [7] Through the use of a modified Krebs-Henseleit buffer, we can uniquely visualize the device-tissue interface: in this study, the placement of catheters.

The purpose of this study was two-fold. First, we demonstrated the long term stability of MAP recordings in an in situ swine model. Second, we showed the relationship between MAPs recorded from in vitro and in situ preparations of each specimen.

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