Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′-diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ~105 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.
- Nanotechnology Institute
Continuous-Flow Microfluidic Devices for Detection of Bacterial Endospores
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Wan, J, Landsman, P, Xia, B, Bower, PE, Heinrich, V, Jones, G, II, & Vullev, VI. "Continuous-Flow Microfluidic Devices for Detection of Bacterial Endospores." Proceedings of the ASME 2006 Frontiers in Biomedical Devices Conference. ASME 2006 Frontiers in Biomedical Devices Conference. Irvine, California, USA. June 8–9, 2006. pp. 23-24. ASME. https://doi.org/10.1115/NANOBIO2006-18030
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