Improving Cell Loading Efficiency Into Microfluidic Devices Using LCATs

Due to the nature of the interfaces used by microfluidic devices — syringes, syringe pumps, and tubing, delivering cells into microfluidic devices faces some practical challenges. Specifically, the unwanted settling and adhesion of cells onto various surfaces can significantly impact the effective transport of cells into the device.[1] One particular challenge is the cell settling that occurs inside devices and especially at the inlet connection port. As most tubing connections are vertical, the cell suspension moves downwards into the device before making a 90° turn into the fluidic channels. This orientation causes cells to settle, clump and adhere to surfaces around the inlet port and eventually causes clogging that prevent more cells from entering the microfluidic channels.