The increasing appreciation of tissue cellular heterogeneity and recent identification of rare cell populations within tissues that are associated with specific biological behaviors, e.g., progenitor cells, has illuminated a limitation of current technologies to study such adherent cells directly from primary tissues. The micropallet array is a recently-developed technology designed to address this limitation by virtue of its capacity to isolate and recover single adherent cells on individual micropallets [1]. Micropallet arrays consist of hundreds of thousands of microscale polymer pedestals (“micropallets”) uniformly arrayed on a glass microscope slide. The micropallets are made from a high aspect photopolymerizable polymer using photolithographic methods. Cells are applied to the arrays and fall stochastically upon its surface, with single cells adhering to individual micropallets. Cells are then analyzed in situ and single, unperturbed cells can be selected and collected from the array by releasing the underlying micropallets using a focused pulsed laser.
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ASME 2010 5th Frontiers in Biomedical Devices Conference
September 20–21, 2010
Newport Beach, California, USA
Conference Sponsors:
- Nanotechnology Institute
ISBN:
978-0-7918-4945-3
PROCEEDINGS PAPER
Novel Microtechnology System for Cytometric Analysis of Adherent Cell Populations
Nicholas M. Gunn,
Nicholas M. Gunn
University of California, Irvine, Irvine, CA
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Mark Bachman,
Mark Bachman
University of California, Irvine, Irvine, CA
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Lifeng Zheng,
Lifeng Zheng
University of California, Irvine, Irvine, CA
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G.-P. Li,
G.-P. Li
University of California, Irvine, Irvine, CA
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Edward L. Nelson
Edward L. Nelson
University of California, Irvine, Irvine, CA
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Nicholas M. Gunn
University of California, Irvine, Irvine, CA
Mark Bachman
University of California, Irvine, Irvine, CA
Lifeng Zheng
University of California, Irvine, Irvine, CA
G.-P. Li
University of California, Irvine, Irvine, CA
Edward L. Nelson
University of California, Irvine, Irvine, CA
Paper No:
BioMed2010-32034, pp. 59-60; 2 pages
Published Online:
July 16, 2013
Citation
Gunn, NM, Bachman, M, Zheng, L, Li, G, & Nelson, EL. "Novel Microtechnology System for Cytometric Analysis of Adherent Cell Populations." Proceedings of the ASME 2010 5th Frontiers in Biomedical Devices Conference. ASME 2010 5th Frontiers in Biomedical Devices Conference and Exhibition. Newport Beach, California, USA. September 20–21, 2010. pp. 59-60. ASME. https://doi.org/10.1115/BioMed2010-32034
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