In some cases an object can not be completely imaged with a single image. These cases call for taking multiple images and stitching the images together to form a complete view of the object. The images that are dealt with are taken with a confocal laser-scanning microscope. This produces a stack of two-dimensional images that represent the volume in the microscope’s field of view. While the microscope automatically creates a z stack, the positioning of the tiles in x and y is done manually. Therefore, the slices within each stack are assumed to be already aligned. Our technique will take multiple stacks of images and stitch them together to form a single volume. We will use a particle swarm optimization technique to calculate the proper transformations required to produce the final volume.
Volumetric Image Alignment Utilizing Particle Swarm Optimization
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Cheng, L, & Meyer, J. "Volumetric Image Alignment Utilizing Particle Swarm Optimization." Proceedings of the ASME 2009 4th Frontiers in Biomedical Devices Conference. ASME 2009 4th Frontiers in Biomedical Devices. Irvine, California, USA. June 8–9, 2009. pp. 75-76. ASME. https://doi.org/10.1115/BioMed2009-83084
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