A modified Mach-Zender set-up in reflection is applied to record and reconstruct holographic amplitude and phase images. A charged couple device (CCD) is used to record a hologram and numerical reconstruction algorithms are then applied to rebuild the hologram for obtaining both phase and amplitude information. One could also focus on multiple focal planes from a single hologram, similar to the focusing control of a conventional microscope. The morphology and behavior of mammalian cells is determined by an interaction between signals from the intracellular matrix and the cellular responses. It is important to note that the physical aspect of the extracellular matrix is as significant as the chemical nature of it. Specifically the stresses, mechanical forces, and the profile of the external environment have major effects on cell behavior. The mechanical and physical characteristics of a tissue are greatly dependent on a hierarchical spatial arrangement of its extra-cellular matrix components. A key player in the ECM is collagen which exhibits significant tensile strength on the cellular scale. Digital holographic microscopy (DHM) is applied to study the deformation of collage matrix in response to cell migration.
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ASME 2007 2nd Frontiers in Biomedical Devices Conference
June 7–8, 2007
Irvine, California, USA
Conference Sponsors:
- Nanotechnology Institute
ISBN:
0-7918-4266-5
PROCEEDINGS PAPER
Measuring the Cell-Induced Deformation of Collagen Matrix Detected With Digital Holographic Microscopy
Kaveh Azartash,
Kaveh Azartash
University of California at Irvine, Irvine, CA
Search for other works by this author on:
Enrico Gratton
Enrico Gratton
University of California at Irvine, Irvine, CA
Search for other works by this author on:
Kaveh Azartash
University of California at Irvine, Irvine, CA
Enrico Gratton
University of California at Irvine, Irvine, CA
Paper No:
BioMed2007-38064, pp. 123-124; 2 pages
Published Online:
February 24, 2009
Citation
Azartash, K, & Gratton, E. "Measuring the Cell-Induced Deformation of Collagen Matrix Detected With Digital Holographic Microscopy." Proceedings of the ASME 2007 2nd Frontiers in Biomedical Devices Conference. ASME 2007 2nd Frontiers in Biomedical Devices. Irvine, California, USA. June 7–8, 2007. pp. 123-124. ASME. https://doi.org/10.1115/BioMed2007-38064
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